Flow Cytometry Facilities
|AIBN researchers have access to an extensive suite of flow cytometry instrumentation.|
Through the AIBN/QBI joint appointment of Dr Geoff Osborne, AIBN researchers have access to an extensive suite of flow cytometry instrumentation.
Instruments and expertise include:
- Cytopeia Influx Cell Sorter: the most advanced cell sorting flow cytometer in the suite of instruments, currently configured with four lasers and nineteen detectors all housed in a Class II certified laminar flow cabinet. This instrument has multi-well plate handling capability for both sample acquisition and deposition making it ideally suited to screening applications of novel compounds on neural cells and selection of subsequent responding cells.
- BD FACSAria Cell Sorter: This high capacity cell sorter fitted with fourteen fluorescent detectors, multi-well plate cell deposition option and aerosol management is ideally placed to fulfill the research needs of scientists requiring more routine multi parametric cell sorting.
- BD FACSVantage SE DiVa Cell Sorter: comprises a three-laser, eight fluorescence detector system capable of sorting for routine applications such as surface markers and DNA binding dyes but also novel particle separations but utilizing nozzles with diameters up to 400um. These large nozzles facilitate the sorting of various neural cell populations that are in some cases, twice as large as those blood cell types that are typically sorted.
- BD LSR II analyzing flow cytometer that can simultaneously detect as many as 18 fluorochromes, which are excited from four individual lasers (UV, 407, 488, 633nm). This instrument increases QBI's analytical capabilities by providing additional excitation wavelengths and making available some of the sorter time that is currently dedicated to analysis.
|Flow Cytometry Facilities section|